Review



s pneumoniae r6 genomic dna  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier
Bioz Manufacturer Symbol Thermo Fisher manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 99
      Buy from Supplier

    Structured Review

    Thermo Fisher s pneumoniae r6 genomic dna
    S. <t> pneumoniae </t> strains used in this study.
    S Pneumoniae R6 Genomic Dna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/s+pneumoniae+r6+genomic+dna/DNA/pmc08727766-212-18-27
    Average 99 stars, based on 1 article reviews
    s pneumoniae r6 genomic dna - by Bioz Stars, 2026-10
    99/100 stars

    Images

    1) Product Images from "The Role of Minor Pilins in Assembly and Function of the Competence Pilus of Streptococcus pneumoniae"

    Article Title: The Role of Minor Pilins in Assembly and Function of the Competence Pilus of Streptococcus pneumoniae

    Journal: Frontiers in Cellular and Infection Microbiology

    doi: 10.3389/fcimb.2021.808601

    S. pneumoniae strains used in this study.
    Figure Legend Snippet: S. pneumoniae strains used in this study.

    Techniques Used:

    Competence pilus assembly and transformability depends on the pneumococcal minor pilins. (A) Schematic representation of the comG operon in S. pneumoniae R6 strain encoding comGA and comGB (white), the major pilin gene comGC (black) and the minor pilin genes comGD, comGE, comGF and comGG (grey). The invariant Glu5 residue, present in all pneumococcal pilins except ComGG, is highlighted (E5). (B) ComGC was detected by immunoblotting in bacterial whole cell lysates and sheared pili of mutants lacking Δ comGC , Δ comGG , Δ comGFG , Δ comGEFG . The WT strain was included as a positive control and GAPDH as loading control. (C) Transformation frequency of WT , comG mutants and R6Δ comGDEFG complemented strains. The detection limit (dl) of the assay was 2.39e -9 and the error bars represent the standard deviation of a minimum of three independent experiments (n=3). (D) Western blotting analysis of ComGC in bacterial whole cell lysates and sheared pili of Δ comGDEFG strains expressing individual minor pilins, comGDEF or comGDEFG at the ectopic bgaA locus. The WT strain was included as a positive control and GAPDH as loading control. (E) Immunofluorescence microscopy (IF) of competence-induced pili in WT R6 and R6Δ comGDEFG complemented with comGG, comGDEF or comGDEFG. Bacteria were visualized by bright field (BF) microscopy and competence pili were labelled with antisera specific for ComGC (red). Scale bars represent 2 µm.
    Figure Legend Snippet: Competence pilus assembly and transformability depends on the pneumococcal minor pilins. (A) Schematic representation of the comG operon in S. pneumoniae R6 strain encoding comGA and comGB (white), the major pilin gene comGC (black) and the minor pilin genes comGD, comGE, comGF and comGG (grey). The invariant Glu5 residue, present in all pneumococcal pilins except ComGG, is highlighted (E5). (B) ComGC was detected by immunoblotting in bacterial whole cell lysates and sheared pili of mutants lacking Δ comGC , Δ comGG , Δ comGFG , Δ comGEFG . The WT strain was included as a positive control and GAPDH as loading control. (C) Transformation frequency of WT , comG mutants and R6Δ comGDEFG complemented strains. The detection limit (dl) of the assay was 2.39e -9 and the error bars represent the standard deviation of a minimum of three independent experiments (n=3). (D) Western blotting analysis of ComGC in bacterial whole cell lysates and sheared pili of Δ comGDEFG strains expressing individual minor pilins, comGDEF or comGDEFG at the ectopic bgaA locus. The WT strain was included as a positive control and GAPDH as loading control. (E) Immunofluorescence microscopy (IF) of competence-induced pili in WT R6 and R6Δ comGDEFG complemented with comGG, comGDEF or comGDEFG. Bacteria were visualized by bright field (BF) microscopy and competence pili were labelled with antisera specific for ComGC (red). Scale bars represent 2 µm.

    Techniques Used: Residue, Western Blot, Positive Control, Control, Transformation Assay, Standard Deviation, Expressing, Immunofluorescence, Microscopy, Bacteria

    Related Articles

    Polymerase Chain Reaction:

    Article Title: The Role of Minor Pilins in Assembly and Function of the Competence Pilus of Streptococcus pneumoniae
    Article Snippet: .. For this, PCR fragments flanking the upstream and downstream regions of the target genes were amplified from the S. pneumoniae R6 genomic DNA using Fusion Flash polymerase (Thermo Fisher Scientific). ..

    Amplification:

    Article Title: The Role of Minor Pilins in Assembly and Function of the Competence Pilus of Streptococcus pneumoniae
    Article Snippet: .. For this, PCR fragments flanking the upstream and downstream regions of the target genes were amplified from the S. pneumoniae R6 genomic DNA using Fusion Flash polymerase (Thermo Fisher Scientific). ..



    Similar Products

    s pneumoniae r6 genomic dna  (Thermo Fisher)
    99
    Thermo Fisher s pneumoniae r6 genomic dna
    S. <t> pneumoniae </t> strains used in this study.
    S Pneumoniae R6 Genomic Dna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/s+pneumoniae+r6+genomic+dna/DNA/pmc08727766-212-18-27
    Average 99 stars, based on 1 article reviews
    s pneumoniae r6 genomic dna - by Bioz Stars, 2026-10
    99/100 stars
    		  Buy from Supplier
    baa 255d 5 s pneumoniae  (ATCC)
    90
    ATCC baa 255d 5 s pneumoniae
    S. <t> pneumoniae </t> strains used in this study.
    Baa 255d 5 S Pneumoniae, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/s+pneumoniae+r6+genomic+dna/Genomic+DNA+from+Streptococcus+pneumoniae+strain+R6/pmc07088346-51-4-8
    Average 90 stars, based on 1 article reviews
    baa 255d 5 s pneumoniae - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    S. pneumoniae strains used in this study.

    Journal: Frontiers in Cellular and Infection Microbiology

    Article Title: The Role of Minor Pilins in Assembly and Function of the Competence Pilus of Streptococcus pneumoniae

    doi: 10.3389/fcimb.2021.808601

    Figure Lengend Snippet: S. pneumoniae strains used in this study.

    Article Snippet: For this, PCR fragments flanking the upstream and downstream regions of the target genes were amplified from the S. pneumoniae R6 genomic DNA using Fusion Flash polymerase (Thermo Fisher Scientific).

    Techniques:

    Competence pilus assembly and transformability depends on the pneumococcal minor pilins. (A) Schematic representation of the comG operon in S. pneumoniae R6 strain encoding comGA and comGB (white), the major pilin gene comGC (black) and the minor pilin genes comGD, comGE, comGF and comGG (grey). The invariant Glu5 residue, present in all pneumococcal pilins except ComGG, is highlighted (E5). (B) ComGC was detected by immunoblotting in bacterial whole cell lysates and sheared pili of mutants lacking Δ comGC , Δ comGG , Δ comGFG , Δ comGEFG . The WT strain was included as a positive control and GAPDH as loading control. (C) Transformation frequency of WT , comG mutants and R6Δ comGDEFG complemented strains. The detection limit (dl) of the assay was 2.39e -9 and the error bars represent the standard deviation of a minimum of three independent experiments (n=3). (D) Western blotting analysis of ComGC in bacterial whole cell lysates and sheared pili of Δ comGDEFG strains expressing individual minor pilins, comGDEF or comGDEFG at the ectopic bgaA locus. The WT strain was included as a positive control and GAPDH as loading control. (E) Immunofluorescence microscopy (IF) of competence-induced pili in WT R6 and R6Δ comGDEFG complemented with comGG, comGDEF or comGDEFG. Bacteria were visualized by bright field (BF) microscopy and competence pili were labelled with antisera specific for ComGC (red). Scale bars represent 2 µm.

    Journal: Frontiers in Cellular and Infection Microbiology

    Article Title: The Role of Minor Pilins in Assembly and Function of the Competence Pilus of Streptococcus pneumoniae

    doi: 10.3389/fcimb.2021.808601

    Figure Lengend Snippet: Competence pilus assembly and transformability depends on the pneumococcal minor pilins. (A) Schematic representation of the comG operon in S. pneumoniae R6 strain encoding comGA and comGB (white), the major pilin gene comGC (black) and the minor pilin genes comGD, comGE, comGF and comGG (grey). The invariant Glu5 residue, present in all pneumococcal pilins except ComGG, is highlighted (E5). (B) ComGC was detected by immunoblotting in bacterial whole cell lysates and sheared pili of mutants lacking Δ comGC , Δ comGG , Δ comGFG , Δ comGEFG . The WT strain was included as a positive control and GAPDH as loading control. (C) Transformation frequency of WT , comG mutants and R6Δ comGDEFG complemented strains. The detection limit (dl) of the assay was 2.39e -9 and the error bars represent the standard deviation of a minimum of three independent experiments (n=3). (D) Western blotting analysis of ComGC in bacterial whole cell lysates and sheared pili of Δ comGDEFG strains expressing individual minor pilins, comGDEF or comGDEFG at the ectopic bgaA locus. The WT strain was included as a positive control and GAPDH as loading control. (E) Immunofluorescence microscopy (IF) of competence-induced pili in WT R6 and R6Δ comGDEFG complemented with comGG, comGDEF or comGDEFG. Bacteria were visualized by bright field (BF) microscopy and competence pili were labelled with antisera specific for ComGC (red). Scale bars represent 2 µm.

    Article Snippet: For this, PCR fragments flanking the upstream and downstream regions of the target genes were amplified from the S. pneumoniae R6 genomic DNA using Fusion Flash polymerase (Thermo Fisher Scientific).

    Techniques: Residue, Western Blot, Positive Control, Control, Transformation Assay, Standard Deviation, Expressing, Immunofluorescence, Microscopy, Bacteria